Isolation of mutants blocked in calicheamicin biosynthesis.
نویسندگان
چکیده
Blocked mutants of Micromonospora echinospora defective in the production of the potent antitumor agent calicheamicin are described. Analysis of intermediates produced by blocked mutants indicates a branched biosynthetic pathway. Mutants that produced some, but not all, calicheamicin forms are also described.
منابع مشابه
Isolation and characterization of furanomycin nonproducing Streptomyces threomyceticus mutants.
Stable mutants that are blocked in the production of the polyketide antibiotic furanomycin were generated by treatment of Streptomyces threomyceticus (ATCC 15795) with a combination of N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and ultraviolet light. On the basis of their cosynthetic properties in mixed culture, the mutants were grouped into eleven phenotypic classes. The polarity of the cosynt...
متن کاملElucidation of structure of novel macrolide antibiotics produced by mutant strains of Streptomyces fradiae.
Tylosin is a 16-membered macrolide antibiotic which is produced commercially by strains of Streptomyces fradiae'•1' and is composed of a substituted lactone (tylonolide), an amino sugar (mycaminose) and two neutral sugars (mycinose, mycarose)'). Recently, a series of mutants of S. fi•adiae which are blocked in specific steps in the biosynthesis of tylosin has been described". From an analysis o...
متن کاملThe Biosynthesis of Histidine
It was later shown (2) that the mutant C84 accumulates imidazoleglycerol’ in the growth medium, that mutant Cl41 accumulates imidazoleglycerol and imidazoleacetol,2 and that T1710 accumulates L-histidinol.3 It was also observed that none of these compounds will replace histidine in promoting the growth of these mutants or other mutants blocked earlier in the sequence. This suggested that these ...
متن کاملBiosynthesis Characteristic of Silver Nanoparticles Produced by Mine Soil Bacteria Isolation, Kerman, Iran
Background: Biosynthesis of is one of the safe methods in nanoparticles (NP) production where local bacteria of every region can be applied for its better functionality. Among these nanoparticles, the silver nanoparticles (SNP) with high applicability due to their antimicrobial characteristic are the most common. Methods: In the study, the soil samples are collected from met...
متن کاملThe isolation of adenylosuccinate synthetase mutants in yeast by selection for constitutive behavior in pigmented strains.
ROMAN’S (1956) selective system for the isolation of yeast mutants blocked in early steps of purine biosynthesis (for review see MAGASANIK 1962) was based on the red color of aerobic &2 and ade2 cells. His method led to the identification of the genetic loci d e 3 through ade8 and stimulated a number of investigations which (1 ) exploit the availability of many independent alleles at specific l...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 173 23 شماره
صفحات -
تاریخ انتشار 1991